E-Portfolio: Microbiology - Week 12

Hi peeps!

This week, Dr Wan taught us more about Viruses, Viriods and Prions. Basically, a virus is an infectious particle that reproduces by commandeering a host cell and using its machinery to make more viruses. A virus is made up of a DNA or RNA genome inside a protein shell called a capsid. Some viruses have an internal or external membrane envelope. Viroid is a piece of RNA without a protein coat responsible for several plant disease. Prions is consist of single type of protein with no nucleic acid component. 

We also were given a group task to find information on a single virus of our choice. My group chose the Rabies virus. Rabies virus is a rod- or bullet-shaped, single-stranded, negative-sense, unsegmented, enveloped RNA virus. The virus genome encodes five proteins. Placement within the family is based on the distinctive morphology of the virus particle. Cross- reactive nucleoprotein antigens or comparative genomic sequences determine inclusion in the genus Lyssavirus, which includes rabies virus and at least five other pathogenic rabies-like viruses. 

Next, for this week lab session. We had done the experiment on Serial Dilution using agar plate technique. Serial dilution a set of dilutions in a mathematical sequence. In microbiological technique, serial dilutions are used to obtain a culture plate that yields a countable number of separate colonies. From this, a calculation of viable cells in the original suspension can be made, as a colony picked for pure culture. Here is some pictures from the experiment: 

Here is a picture of Fatin counting the colonies using Quebec colony counter.

E. coli colonies on the agar plate from dilution

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