E-Portfolio Microbiology II Week 12

Assalam all, so this week actually we do not have any BMY class due to the General Election 14 on 8th of May, Wednesday. Hope to update more to you guys soon. Bye :0

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E-Portfolio: Microbiology - Week 7

Imej
What is up, people! This week, we learned on internal structure and its function of a prokaryote with Dr. Wan Courtesy: https://www.shmoop.com/biology-cells/prokaryotic-cells.html I am actually quiet interested with plasmid, a small structure, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. Plasmids naturally exist in bacterial cells, and they also occur in some eukaryotes. Often, the genes carried in plasmids provide bacteria with genetic advantages, such as antibiotic resistance. Plasmids have a wide range of lengths, from roughly one thousand DNA base pairs to hundreds of thousands of base pairs. When a bacterium divides, all of the plasmids contained within the cell are copied such that each daughter cell receives a copy of each plasmid. Bacteria can also transfer plasmids to one another through a process called conjugation using sex pili. For our experiment this week, we had done Gram staining and Acid-fast staining.
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E-Portfolio BMY3201 - Experiment 2 & 3

Imej
27th September 2017 This week I learned on several techniques for microbes culturing that involve plating, using nutrient broth and agar, also using gelatin. There are few types of plating such as streak plate method, spread plate method and pour plate method. I managed to practices on doing streak-plate and spread-plate method. For streak-plate method, I learned on how to use inoculation loop by streaking on the agar plate surface. It is quite hard but I think I can improve my techniques in the future. For spread-plate method, we basically use bent glass rod to spread the microbes on the surface of agar plate, but for this technique we use alcohol to sterilize the glass rod which is actually quite harmful as we also use bunser burner for the same experiment. We must practice proper precaution and handle the experiment carefully to avoid any accident. Here is some pictures from this week experiment : Microorganism sample from toilet sink E. coli streaking culture
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